This study aimed to evaluate the antioxidant activity and toxicity of the ethanolic extract of red mangrove leaves (Sonneratia caseolaris). Extraction was conducted by maceration using 96% ethanol. Antioxidant activity was tested using the DPPH and ABTS methods with vitamin C as a reference, while toxicity was determined through the Brine Shrimp Lethality Test (BSLT). The extract showed very strong antioxidant activity with IC50 values of 37.67 ppm (ABTS) and 4.25 ± 3.05 ppm (DPPH). LC-HRMS identified TEMPO, Choline, and Betaine, with TEMPO showing the highest antioxidant potential. The BSLT result indicated non-toxic properties (LC50 >1500 ppm). The extract shows promise as a safe and effective natural antioxidant source.

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