ISOLATION OF ARBUTIN FROM SUKUN (ARTOCARPUS ALTILIS [PARK.] FOSBERG.) LEAF AND STEM EXTRACTS AS A TYROSINASE INHIBITOR
Abstract
This study aims to isolate arbutin compounds from 70% ethanol extract of breadfruit (Artocarpus altilis) leaves and stems, determine the levels of arbutin in the extract, and evaluate the inhibitory activity of tyrosinase enzyme. Isolation was carried out through extraction and fractionation methods using n-hexane, ethyl acetate, and butanol solvents. Identification of arbutin was carried out by High Performance Liquid Chromatography (HPLC) based on the suitability of retention time to the standard. The results showed that the level of arbutin in 70% ethanol extract of breadfruit leaves was 14.84 mg/L and breadfruit stems was 14.81 mg/L. Tyrosinase activity test showed that ethyl acetate fraction of breadfruit leaves had the highest effectiveness as tyrosinase inhibitor with IC₅₀ value of 355.24 ppm, compared to crude extract and other fractions. Although the activity has not matched the positive control kojic acid (IC₅₀ = 39.28 ppm), these results indicate the potential of breadfruit leaf and stem extracts as a source of natural active ingredients for skin lightening
Keywords
Full Text:
PDFReferences
Aji, S. P., Nurhayati, N., & Purwaningsih, E. H. (2020). Aktivitas antioksidan dan penghambatan enzim tirosinase dari ekstrak daun sukun (Artocarpus altilis) sebagai bahan pencerah kulit. Jurnal Kimia dan Pendidikan Kimia, 5(1), 25–32.
Hasibuan, S. N., Sitorus, P., & Hutahaean, S. (2020). Risiko penggunaan bahan berbahaya dalam kosmetik pencerah kulit: Studi literatur hidrokuinon dan merkuri. Jurnal Farmasi Indonesia, 17(2), 101–108.
Lee, Y. M., Kim, Y. K., & Park, J. H. (2021). Comparative study of α-arbutin and β-arbutin as tyrosinase inhibitors in skin-whitening cosmetics. Journal of Cosmetic Dermatology, 20(2), 489–495. https://doi.org/10.1111/jocd.13734
Pratiwi, A. D., Ramadaniyah, N., & Hidayati, R. N. (2022). Uji aktivitas penghambatan enzim tirosinase dari ekstrak etanol 70% daun sukun (Artocarpus altilis). Pharmaciana, 12(1), 73– 80.
Putra, A. R., Syafitri, U. D., & Lestari, N. D. (2021). Aktivitas ekstrak batang sukun (Artocarpus altilis) sebagai penghambat tirosinase. Jurnal Fitofarmaka Indonesia, 8(2), 85–90.
Sari, P. N., & Handayani, T. (2021). Klasifikasi aktivitas penghambatan tirosinase dari bahan alam berdasarkan nilai IC₅₀. Jurnal Ilmu Kefarmasian Indonesia, 19(1), 45–52.
Surini, S., Mardliyati, E., & Oktaviani, D. (2019). Efektivitas formulasi gel pencerah kulit mengandung arbutin dan vitamin C terhadap hiperpigmentasi kulit akibat sinar UV. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 5(2), 162–171.
Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. (2018). Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and cosmetic applications. Applied Sciences, 8(3), 319. https://doi.org/10.3390/app8030319
DOI: https://doi.org/10.52447/inrpj.v10i2.9220
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright@ Pusat Penelitian Fakultas Farmasi
Universitas 17 Agustus 1945 Jakarta
Online ISSN : 2502-8421