NARRATIVE REVIEW : METODE EKSTRAKSI GANODERMA LUCIDUM DAN EFEKTIVITASNYA

Meinanda Grabilia; Diana Laila Rahmatillah; Kashif Khan

doi:10.52447/inrpj.v11i1.9048

NARRATIVE REVIEW : METODE EKSTRAKSI GANODERMA LUCIDUM DAN EFEKTIVITASNYA

Meinanda Grabilia, Diana Laila Rahmatillah, Kashif Khan

Abstract

Ganoderma lucidum is one of the medicinal mushrooms that has been extensively researched due to its bioactive compounds, particularly polysaccharides, triterpenoids, and phenolic compounds, which exhibit a wide range of pharmacological activities. Various extraction methods have been developed to optimally obtain these bioactive components, ranging from conventional techniques like hot water and ethanol extraction to modern methods such as ultrasonic extraction, microwave-assisted extraction, and enzyme-based combinations. This review article aims to identify the types of extraction methods used on G. lucidum and evaluate their effectiveness based on the resulting active compound composition and biological activity. The review results indicate that differences in solvents, temperature, duration, and processing techniques significantly affect the yield and stability of bioactive components. The extracted compounds have been proven to possess antioxidant, antimicrobial, hepatoprotective, immunomodulatory, and antitumor activities, although their effectiveness varies across studies due to the lack of standardisation in the extraction process. This review emphasises the importance of selecting the appropriate extraction method based on the target compounds, as well as the need for further research to standardise the process and quantitatively compare the effectiveness of the methods. Thus, the therapeutic potential of G. lucidum can be utilised more optimally in the development of herbal-based health and pharmaceutical products.

Keywords: Ganoderma lucidum, extraction method, bioactive, effectiveness

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References

Agustin, F., Toharmat, T., Evvyernie, D., Taniwiryono, D., Tarigan, S., & Prihantoro, I. (2010). Chromium incorporation by Ganoderma lucidum with oil palm by-product as substrate | Inkorporasi kromium oleh fungi Ganoderma lucidum dengan limbah industri kelapa sawit sebagai substrat. Media Peternakan, 33(1), 18–24.

Ahmad, M. F., A. Alsayegh, A., Ahmad, F. A., Akhtar, M. S., Alavudeen, S. S., Bantun, F., Wahab, S., Ahmed, A., Ali, M., Elbendary, E. Y., Raposo, A., Kambal, N., & H. Abdelrahman, M. (2024). Ganoderma lucidum: Insight into antimicrobial and antioxidant properties with development of secondary metabolites. Heliyon, 10(3). https://doi.org/10.1016/j.heliyon.2024.e25607

Ahmad, M. F., Ahmad, F. A., Hasan, N., Alsayegh, A. A., Hakami, O., Bantun, F., Tasneem, S., Alamier, W. M., Babalghith, A. O., Aldairi, A. F., Kambal, N., & Elbendary, E. Y. (2024). Ganoderma lucidum: Multifaceted mechanisms to combat diabetes through polysaccharides and triterpenoids: A comprehensive review. International Journal of Biological Macromolecules, 268(January). https://doi.org/10.1016/j.ijbiomac.2024.131644

Ahmad, M. F., Ahmad, F. A., Zeyaullah, M., Alsayegh, A. A., Mahmood, S. E., AlShahrani, A. M., Khan, M. S., Shama, E., Hamouda, A., Elbendary, E. Y., & Attia, K. A. H. A. (2023). Ganoderma lucidum: Novel Insight into Hepatoprotective Potential with Mechanisms of Action. Nutrients, 15(8). https://doi.org/10.3390/nu15081874

Ahmad, R., Riaz, M., Khan, A., Aljamea, A., Algheryafi, M., Sewaket, D., & Alqathama, A. (2021). Ganoderma lucidum (Reishi) an edible mushroom; a comprehensive and critical review of its nutritional, cosmeceutical, mycochemical, pharmacological, clinical, and toxicological properties. Phytotherapy Research, 35(11), 6030–6062. https://doi.org/10.1002/ptr.7215

Alshiekheid, M. A., Umar, A., Ameen, F., Alyahya, S. A., & Dufossé, L. (2023). Biodegradation of chromium by laccase action of Ganoderma multipileum. Journal of King Saud University - Science, 35(10). https://doi.org/10.1016/j.jksus.2023.102948

Aly Farag El Sheikha. (2022). Nutritional Profile and Health Benefits of Ganoderma lucidum “Lingzhi, Reishi, or Mannentake” as Functional Foods: Current Scenario and Future Perspectives. Foods, 11, 1–29.

Alzorqi, I., Sudheer, S., Lu, T. J., & Manickam, S. (2017). Ultrasonically extracted β-Dglucan from artificially cultivated mushroom, characteristic properties and antioxidant activity. Ultrasonics Sonochemistry, 35, 531–540. https://doi.org/10.1016/j.ultsonch.2016.04.017

Andrejc, D. C., Knez, Z., & Marevci, M. K. (2022). Antioxidant, antibacterial, antitumor, antifungal, antiviral, anti-inflammatory, and nevro-protective activity of Ganoderma lucidum:An overview. frontliers in Pharmocology, 22(July), 1–14. https://doi.org/10.3389/fphar.2022.934982

Baby, S., Johnson, A. J., & Govindan, B. (2015). Secondary metabolites from Ganoderma. Phytochemistry, 114, 66–101. https://doi.org/10.1016/j.phytochem.2015.03.010

Bishop, K. S., Kao, C. H. J., Xu, Y., Glucina, M. P., Paterson, R. R. M., & Ferguson, L. R. (2015). From 2000 years of Ganoderma lucidum to recent developments in nutraceuticals. Phytochemistry, 114(2), 56–65. https://doi.org/10.1016/j.phytochem.2015.02.015

Blundell, R., Camilleri, E., Baral, B., Karpiński, T. M., Neza, E., & Atrooz, O. M. (2023). The Phytochemistry of Ganoderma Species and their Medicinal Potentials. American Journal of Chinese Medicine, 51(4), 859–882. https://doi.org/10.1142/S0192415X23500404

Boh, B., Berovic, M., Zhang, J., & Zhi-Bin, L. (2007). Ganoderma lucidum and its pharmaceutically active compounds. In Biotechnology Annual Review (Vol. 13, Nomor 07). https://doi.org/10.1016/S1387-2656(07)13010-6

Cadar, E., Negreanu-Pirjol, T., Pascale, C., Sirbu, R., Prasacu, I., Negreanu-Pirjol, B. S., Tomescu, C. L., & Ionescu, A. M. (2023). Natural Bio-Compounds from Ganoderma lucidum and Their Beneficial Biological Actions for Anticancer Application: A Review. Antioxidants, 12(11). https://doi.org/10.3390/antiox12111907

Cancemi, G., Caserta, S., Gangemi, S., Pioggia, G., & Allegra, A. (2024). Exploring the Therapeutic Potential of Ganoderma lucidum in Cancer. Journal of Clinical Medicine, 13(4). https://doi.org/10.3390/jcm13041153

Chen, S., Guan, X., Yong, T., Gao, X., Xiao, C., Xie, Y., Chen, D., Hu, H., & Wu, Q. (2022). Structural characterization and hepatoprotective activity of an acidic polysaccharide from Ganoderma lucidum. Food Chemistry: X, 13(September 2021), 100204. https://doi.org/10.1016/j.fochx.2022.100204

Chen, X. J., Deng, Z., Zhang, L. Le, Pan, Y., Fu, J., Zou, L., Bai, Z., Xiao, X., & Sheng, F. (2024). Therapeutic potential of the medicinal mushroom Ganoderma lucidum against Alzheimer’s disease. Biomedicine and Pharmacotherapy, 172(October 2023), 116222. https://doi.org/10.1016/j.biopha.2024.116222

Constantin, M., Iuliana, R., Suica-bunghez, R., Firinca, C., Radu, N., Gurban, A., Preda, S., Alexandrescu, E., Doni, M., & Jecu, L. (2023). Ganoderma lucidum-mediated green synthesis of silver nanoparticles with antimicrobial activity. Materials, 16(12), 4261. https://doi.org/10.3390/ma16124261

Du, Y., Tian, L., Wang, Y., Li, Z., & Xu, Z. (2024). Chemodiversity, pharmacological activity, and biosynthesis of specialized metabolites from medicinal model fungi Ganoderma lucidum. Chinese Medicine (United Kingdom), 19(1), 74. https://doi.org/10.1186/s13020-024-00922-0

Ekiz, E., Oz, E., Abd El-Aty, A. M., Proestos, C., Brennan, C., Zeng, M., Tomasevic, I., Elobeid, T., Çadırcı, K., Bayrak, M., & Oz, F. (2023). Exploring the potential medicinal benefits of Ganoderma lucidum: From metabolic disorders to coronavirus infections. Foods, 12(7), 1512. https://doi.org/10.3390/foods12071512

Galappaththi, M. C. A., Patabendige, N. M., Premarathne, B. M., Hapuarachchi, K. K., Tibpromma, S., Dai, D. Q., Suwannarach, N., Rapior, S., & Karunarathna, S. C. (2023). A review of Ganoderma triterpenoids and their bioactivities. Biomolecules, 13(1), 24. https://doi.org/10.3390/biom13010024

Gao, X., & Homayoonfal, M. (2023). Exploring the anti-cancer potential of Ganoderma lucidum polysaccharides (GLPs) and their versatile role in enhancing drug delivery systems: A multifaceted approach to combat cancer. Cancer Cell International, 23(1), 259. https://doi.org/10.1186/s12935-023-03146-8

He, J., Han, X., Luo, Z. L., Li, E. X., Tang, S. M., Luo, H. M., Niu, K. Y., Su, X. J., & Li, S. H. (2022). Species diversity of Ganoderma (Ganodermataceae, Polyporales) with three new species and a key to Ganoderma in Yunnan Province, China. Frontiers in Microbiology, 13, 1035434. https://doi.org/10.3389/fmicb.2022.1035434

Johra, F. T., Hossain, S., Jain, P., Bristy, A. T., Emran, T., Ahmed, R., Sharker, S. M., Bepari, A. K., & Reza, H. M. (2023). Amelioration of CCl4-induced oxidative stress and hepatotoxicity by Ganoderma lucidum in long evans rats. Scientific Reports, 13(1), 10912. https://doi.org/10.1038/s41598-023-35228-y

Kolniak-Ostek, J., Oszmiański, J., Szyjka, A., Moreira, H., & Barg, E. (2022). Anticancer and antioxidant activities in Ganoderma lucidum wild mushrooms in Poland, as well as their phenolic and triterpenoid compounds. International Journal of Molecular Sciences, 23(16), 9359. https://doi.org/10.3390/ijms23169359

Listriyani, L. W., Hudiyono, S., Zulaeha, S., & Wibisana, A. (2023). Optimization of enzyme-microwave assisted extraction, characterization, and antioxidant activity of polysaccharide from Ganoderma lucidum. Jurnal Bioteknologi & Biosains Indonesia, 10(1), 1–16. http://ejurnal.bppt.go.id/index.php/JBBI

Liu, H. M., Cheng, J., Wang, X. Y., Jiang, Y., Ni, J., Zhang, Y., & Wang, W. (2024). Structure identification of Ganoderma lucidum spore polysaccharides and their antitumor activity in vivo. Molecules, 29(10), 2348. https://doi.org/10.3390/molecules29102348

Liu, J., Zhang, B., Wang, L., Li, S., Long, Q., & Xiao, X. (2024). Bioactive components, pharmacological properties and underlying mechanism of Ganoderma lucidum spore oil: A review. Chinese Herbal Medicines, 16(3), 375–391. https://doi.org/10.1016/j.chmed.2023.09.007

Liu, X., Yang, L., Li, G., Jiang, Y., Zhang, G., & Ling, J. (2023). A novel promising neuroprotective agent: Ganoderma lucidum polysaccharide. International Journal of Biological Macromolecules, 229, 168–180. https://doi.org/10.1016/j.ijbiomac.2022.12.276

Liu, Y., Long, Y., Liu, H., Lan, Y., Long, T., Kuang, R., Wang, Y., & Zhao, J. (2022). Polysaccharide prediction in Ganoderma lucidum fruiting body by hyperspectral imaging. Food Chemistry: X, 13, 100199. https://doi.org/10.1016/j.fochx.2021.100199

Łysakowska, P., Sobota, A., Wirkijowska, A., & Zarzycki, P. (2024). The impact of Ganoderma lucidum (Curtis) P. Karst. supplementation on the technological, chemical, and quality parameters of wheat bread. Foods, 13(19), 3101. https://doi.org/10.3390/foods13193101

Matsunaga, Y., Machmudah, S., Wahyudiono, Kanda, H., Sasaki, M., & Goto, M. (2014). Subcritical water extraction and direct formation of microparticulate polysaccharide powders from ganoderma lucidum. International Journal of Technology, 5(1), 40–50. https://doi.org/10.14716/ijtech.v5i1.152

Meng, M., Yao, J., Zhang, Y., Sun, H., & Liu, M. (2023). Potential anti-rheumatoid arthritis activities and mechanisms of Ganoderma lucidum polysaccharides. Molecules, 28(6), 2483. https://doi.org/10.3390/molecules28062483

Mousavi, S. M., Hashemi, S. A., Gholami, A., Omidifar, N., Chiang, W. H., Neralla, V. R., Yousefi, K., & Shokripour, M. (2023). Ganoderma lucidum methanolic extract as a potent phytoconstituent: Characterization, in-vitro antimicrobial and cytotoxic activity. Scientific Reports, 13(1), 15008. https://doi.org/10.1038/s41598-023-44135-1

Mustafin, K., Bisko, N., Blieva, R., Al-Maali, G., Krupodorova, T., Narmuratova, Z., Saduyeva, Z., & Zhakipbekova, A. (2022). Antioxidant and antimicrobial potential of Ganoderma lucidum and Trametes versicolor. Turkish Journal of Biochemistry, 47(4), 483–489. https://doi.org/10.1515/tjb-2021-0141

Ni, H., Fu, W., Wei, J., Zhang, Y., Chen, D., Tong, J., Chen, Y., Liu, X., Luo, Y., & Xu, T. (2023). Non-destructive detection of polysaccharides and moisture in Ganoderma lucidum using near-infrared spectroscopy and machine learning algorithm. LWT, 184, 115001. https://doi.org/10.1016/j.lwt.2023.115001

Paterson, R. R. M. (2006). Ganoderma - A therapeutic fungal biofactory. Phytochemistry, 67(18), 1985–2001. https://doi.org/10.1016/j.phytochem.2006.07.004

Porter, D. L., Hotz, E. C., Uehling, J. K., & Naleway, S. E. (2023). A review of the material and mechanical properties of select Ganoderma fungi structures as a source for bioinspiration. Journal of Materials Science, 58(8), 3401–3420. https://doi.org/10.1007/s10853-023-08214-y

Raza, S. H. A., Zhong, R., Li, X., Pant, S. D., Shen, X., BinMowyna, M. N., Luo, L., & Lei, H. (2024). Ganoderma lucidum triterpenoids investigating their role in medicinal applications and genomic protection. The Journal of Pharmacy and Pharmacology, 76(12), 1535–1551. https://doi.org/10.1093/jpp/rgae133

Ren, S., Liu, H., Sang, Q., Lu, M., Gao, Q., & Chen, W. (2025). A review of bioactive components and pharmacological effects of Ganoderma lucidum. Food Science and Nutrition, 13(7), 1–19. https://doi.org/10.1002/fsn3.70623

Rijia, A., Krishnamoorthi, R., Rasmi, M., Mahalingam, P. U., & Kim, K. S. (2024). Comprehensive analysis of bioactive compounds in wild Ganoderma applanatum mushroom from Kerala, South India: Insights into dietary nutritional, mineral, antimicrobial, and antioxidant activities. Pharmaceuticals, 17(4), 509. https://doi.org/10.3390/ph17040509

Senphan, T., Benjakul, S., Sukketsiri, W., & Chotphruethipong, L. (2024). Comparative studies on characterizations and cytotoxicity of oil extracted from Lingzhi (Ganoderma lucidum) G2 spore using Soxhlet extraction and microwave-assisted extraction. Applied Food Research, 4(2), 100483. https://doi.org/10.1016/j.afres.2024.100483

Seweryn, E., Ziała, A., & Gamian, A. (2021). Health-promoting of polysaccharides extracted from Ganoderma lucidum. Nutrients, 13(8), 2725. https://doi.org/10.3390/nu13082725

Shen, L., Pang, S., Zhong, M., Sun, Y., Qayum, A., Liu, Y., Rashid, A., Xu, B., Liang, Q., Ma, H., & Ren, X. (2023). A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies. Ultrasonics Sonochemistry, 101, 106646. https://doi.org/10.1016/j.ultsonch.2023.106646

Sunaryanto, R., & Nuraini, D. (2019). Response surface optimization of medium fermentation for Streptomyces prasinopilosus as an antifungal against Ganoderma boninense. Jurnal Bioteknologi & Biosains Indonesia, 6(2), 156–166.

Umar, A., & Ahmed, S. (2022). Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship. Scientific Reports, 12(1), 2416. https://doi.org/10.1038/s41598-022-06111-z

Verma, T., Verma, R., Thawait, R., Kumar, A., Wamankar, S., & Kumar Nema, R. (2024). Ganoderma Lucidum: A comprehensive review of its health benefits and therapeutic potential. International Journal of Advanced Multidisciplinary Research and Studies, 4(6), 1251–1257. https://doi.org/10.62225/2583049x.2024.4.6.3593

Wu, P., Zhang, C., Yin, Y., Zhang, X., Li, Q., Yuan, L., Sun, Y., Zhou, S., Ying, S., & Wu, J. (2024). Bioactivities and industrial standardization status of Ganoderma lucidum: A comprehensive review. Heliyon, 10(19), e36987. https://doi.org/10.1016/j.heliyon.2024.e36987

Wu, S., Zhang, S., Peng, B., Tan, D., Wu, M., Wei, J. C., Wang, Y. T., & Luo, H. (2024). Ganoderma lucidum: A comprehensive review of phytochemistry, efficacy, safety and clinical study. Food Science and Human Wellness, 13(2), 568–596. https://doi.org/10.26599/FSHW.2022.9250051

Wu, Z. W., Zhao, X. F., Quan, C. X., Liu, X. C., Tao, X. Y., Li, Y. J., Peng, X. R., & Qiu, M. H. (2025). Structure–function insights of natural Ganoderma polysaccharides: Advances in biosynthesis and functional food applications. Natural Products and Bioprospecting, 15(1), 28. https://doi.org/10.1007/s13659-025-00496-w

Ye, T., Ge, Y., Jiang, X., Song, H., Peng, C., & Liu, B. (2023). A review of anti-tumour effects of Ganoderma lucidum in gastrointestinal cancer. Chinese Medicine (United Kingdom), 18(1), 121. https://doi.org/10.1186/s13020-023-00811-y

Zhang, H., Zhang, J., Liu, Y., & Tang, C. (2023). Recent advances in the preparation, structure, and biological activities of β-glucan from Ganoderma species: A review. Foods, 12(15), 2975. https://doi.org/10.3390/foods12152975

Zhao, Y., Li, Q., Wang, M., Wang, Y., Piao, C., Yu, H., Liu, J., & Li, Z. (2023). Structural characterization of polysaccharides after fermentation from Ganoderma lucidum and its antioxidant activity in HepG2 cells induced by H2O2. Food Chemistry: X, 18, 100682. https://doi.org/10.1016/j.fochx.2023.100682

Zheng, S., Zhang, W., & Liu, S. (2020). Optimization of ultrasonic-assisted extraction of polysaccharides and triterpenoids from the medicinal mushroom Ganoderma lucidum and evaluation of their in vitro antioxidant capacities. PLOS ONE, 15(12), e0244749. https://doi.org/10.1371/journal.pone.0244749

Zheng, W., Lan, S., Zhang, W., Nie, B., Zhu, K., Ye, X., Hou, Z., & Chen, S. (2024). Polysaccharide structure evaluation of Ganoderma lucidum from different regions in China based on an innovative extraction strategy. Carbohydrate Polymers, 335, 122079. https://doi.org/10.1016/j.carbpol.2024.122079

Zhong, Y., Tan, P., Lin, H., Zhang, D., Chen, X., Pang, J., & Mu, R. (2024). A review of Ganoderma lucidum polysaccharide: Preparations, structures, physicochemical properties and application. Foods, 13(17), 2665. https://doi.org/10.3390/foods13172665

DOI: https://doi.org/10.52447/inrpj.v11i1.9048