REVIEW ARTIKEL : VALIDASI METODE DENGAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DALAM ANALISIS SENYAWA KURKUMIN

Silviani Rahayu; Siti Latifah Hanum; Rafi Dafa Azhari; Ayu Anastasya; Aidah Intan Azkiyah; Lintang Asri

doi:10.52447/inrpj.v10i2.9201

REVIEW ARTIKEL : VALIDASI METODE DENGAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT) DALAM ANALISIS SENYAWA KURKUMIN

Silviani Rahayu, Siti Latifah Hanum, Rafi Dafa Azhari, Ayu Anastasya, Aidah Intan Azkiyah, Lintang Asri

Abstract

Validasi metode merupakan suatu tindakan penilaian terhadap parameter tertentu, berdasarkan percobaan laboratorium untuk membuktikan bahwa parameter tersebut memenuhi persyaratan penggunaannya. Review artikel ini bertujuan untuk menilai tingkat mutu baik suatu bahan ataupun sediaan farmasi dengan menggunakan parameter validasi metode pada alat Kromatografi Cair Kinerja Tinggi (KCKT) untuk mencari senyawa Kurkumin. Kajian ini diterapkan dengan melakukan pemilihan dan pencarian literatur dengan menelusuri beberapa jurnal penelitian dari skala nasional maupun internasional yang dibatasi pada publikasi selama 10 tahun terakhir (2016-2025) melalui basis data elektronik seperti Google Scholar, Pubmed, ScienceDirect, Mendeley berdasarkan kriteria inklusi dan eksklusi dengan menggunakan kata kunci yang relevan dengan topik penelitian. Artikel yang sudah dipilih dianalisis menggunakan data hasil analisis kualitatif dan kuantitatif dengan fokus parameter validasi metode, meliputi akurasi, spesifisitas, presisi, linieritas, batas deteksi (LOD), batas kuantitasi (LOQ). Hasil review artikel ini menunjukkan bahwa Kromatografi Cair Kinerja Tinggi (KCKT) mampu memisahkan kurkumin dari matriks yang rumit dengan nilai akurasi tinggi dengan rentang pemulihan sebesar 98% - 102% serta presisi yang bagus dengan nilai %RSD ≤ 2%, sehingga sangat cocok digunakan untuk pengendalian kualitas produk yang mengandung kurkumin.

Keywords

Validasi metode, Kromatografi Cair Kinerja Tinggi (KCKT), Kurkumin

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References

Yuniar Dwi Nanda, A., Khalida Hanum, N., Nurfadhila, L., Rahmawati Utami, M., Studi Farmasi, P., Ilmu Kesehatan, F., Singaperbangsa Karawang, U., & Barat, J. (2024). Journal of Pharmaceutical and Sciences |Volume 6|No. https://www.journal-jps.com

V. Vernanda, N. L. Buni, D. Tabuni, and B. Wenda, "Analisis Penggunaan Variasi Metode Pengeringan terhadap Persentase Kadar Air dan Kecepatan Proses Pengeringan pada Curcuma zedoaria | JURNAL HOLAN https://holan.unaim-wamena.ac.id/index.php/holan/article/view/1

Hirlekar, R., Khismatrao, A., Bhairy, S. 2018. Development And Validation Of RP-HPLC Method For Simultaneous Estimation Of Curcumin And Piperine.Innovare Academic Sciences Pvt. Ltd. India. 10,Issue-5. DOI: http://dx.doi.org/10.22159/ijap.2018v10i5.21140

Farida, I. N., & Muliya, A. (2023). Validasi Kurkumin Hasil Isolasi Rimpang Kunyit Dengan Kromatografi Cair Kinerja Tinggi Photodiode Array Detector. Jurnal Pengelolaan Laboratorium Pendidikan, 5(2), 50-57. https://doi.org/10.14710/jplp.5.2.50-57

Angraini, N., & Desmaniar, P. (2020). Optimasi penggunaan High Performance Liquid Chromatography (HPLC) untuk analisis asam askorbat guna menunjang kegiatan Praktikum Bioteknologi Kelautan. Jurnal Penelitian Sains, 22(2), 69-75. https://doi.org/10.56064/jps.v22i2.583

Hanwar, D., Handayani, V. R., & Suhendi, A. (2020). Validasi Metode HPLC untuk Analisis Kurkumin pada Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.). Prosiding University Research Colloquium, 371–378. Retrieved from https://repository.urecol.org/index.php/proceeding/article/view/1207

Lee, Y. S., Oh, S. M., Li, Q. Q., Kim, K. W., Yoon, D., Lee, M. H., Kwon, D. Y., Kang, O. H., & Lee, D. Y. (2022). Validation of a Quantification Method for Curcumin Derivatives and Their Hepatoprotective Effects on Nonalcoholic Fatty Liver Disease. Current Issues in Molecular Biology, 44(1), 409–432. https://doi.org/10.3390/cimb44010029

Qirom, A. K. K., Marliani, L., & Kusriani, R. H. (2017). HPLC FINGERPRINT ANALYSIS EKSTRAK DAN PRODUK RIMPANG TEMU PUTIH (Curcuma zedoaria (Christin) Roscoe). JURNAL FARMASI GALENIKA, 4(Edisi Khus), 1–7. Retrieved from https://journal.bku.ac.id/jfg/jfg/article/view/78

Rifai, B., Ihsan, P., Farmasi, J., Kedokteran, F., Brawijaya, U., Malang, J. V., Nurhayati, I. P., & Maysaroh, I. (2023). PHARMACEUTICAL JOURNAL OF INDONESIA Validasi Metode Ultra High Performance Chromatography Double Mass Spectrometry (UHPLC-MS/MS) untuk Analisis Kurkumin pada Ekstrak Etanol Kunyit (Curcuma longa) dengan Berbagai Perbandingan Method Validation of Ultra High Performance Chromatography-Double Mass Spectrometry (UHPLC-MS/MS) for Analysis of Curcumin in Ethanol Extract of Turmeric (Curcuma longa) with Various Comparisons. In PHARMACEUTICAL JOURNAL OF INDONESIA (Vol. 2018, Issue 1). https://share.google/QDrIEA1LuaHMYXLts

Choliq, R. I., Hardani, T., & Sinulingga, A. S. (2020). Journal of Pharmaceutical and Sciences Method Validation of Curcumin Content Determination in Curcuma Rhizome Extract Tablets (Curcuma xanthorrhiza Roxb.) by HPLC Validasi Metode Penetapan Kadar Kurkumin pada Tablet Ekstrak Rimpang Temulawak (Curcuma xanthorrhiza Roxb.) dengan KCKT. JPS, 2024(4), 571–579. https://doi.org/10.36490/journal-jps.com

Salsabila, S. (2023). Penetapan Kadar Kurkumin Metode HPLC dengan Variasi Metode Ekstraksi Maserasi dan Remaserasi pada Ekstrak Rimpang Tanaman Kunyit (Curcuma domestica) [Tugas Akhir, Universitas Brawijaya]. Repository Universitas Brawijaya. https://pji.ub.ac.id/index.php/pji/article/view/84

Desai, N., Momin, M., Singh, U., Khan, T., & Sherje, A. (2019). Analytical method development and validation for simultaneous estimation of curcumin and cyclosporine by RP-HPLC. International Journal of Pharmacy and Pharmaceutical Sciences, 11(2), 26–33. https://doi.org/10.22159/ijpps.2019v11i2.28975

Raut, R., & Shaji, J. (2021). HPLC method validation for quantification of tetrahydrocurcumin in bulk drug and formulation. Future Journal of Pharmaceutical Sciences, 7, 42. https://doi.org/10.1186/s43094-021-00194-7

Noshadi, B., Burgaz, E. V., & Beba Pozharani, L. (2025). Development and Validation of an RP-HPLC Method for the Simultaneous Quantification of Metformin and Curcumin. Cyprus Journal of Medical Sciences, 4–7. https://doi.org/10.4274/cjms.2024.2024-132

Sap, A., & Yaman, M. (2022). Optimization and Validation of HPLC-DAD Method for the Quantification of Curcuminoids in Turmeric (Curcuma Longa L.). J. Anal. Environ. Chem, 23(1), 93–101. https://doi.org/10.21743/pjaec/2022.06.09

Jagtap, P., Mahajan, N., Parte, A., Pananchery, J., & Jain, A. (2021). RP-HPLC Method Development and Validation for Simultaneous Estimation of Thymoquinone and Curcumin in Dosage form. Journal of Pharmaceutical Research International, 53–62. https://doi.org/10.9734/jpri/2021/v33i59b34352

Amanolahi, F., Mohammadi, A., Oskuee, R. K., Nassirli, H., & Malaekeh-Nikouei, B. (2017). A simple, sensitive and rapid isocratic reversed-phase high-performance liquid chromatography method for determination and stability study of curcumin in pharmaceutical samples. Avicenna Journal of Phytomedicine, 7(5), 444–453. https://ajp.mums.ac.ir/article_8861.html

Haq, N., Shakeel, F., Ghoneim, M. M., Asdaq, S. M. B., Alam, P., Alanazi, S. A., & Alshehri, S. (2023). Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and Curcuma longa L. Extract. Separations, 10(2). https://doi.org/10.3390/separations10020098

Nadaf, S., & Killedar, S. (2020). Development and validation of RP–HPLC method for estimation of curcumin from nanocochleates and its application in in–vivo pharmacokinetic study. Acta Chimica Slovenica, 67(4), 1100–1110. https://doi.org/10.17344/acsi.2020.5892

Fonseca-Santos, B., Gremião, M. P. D., & Chorilli, M. (2017). A simple reversed phase high-performance liquid chromatography (HPLC) method for determination of in situ gelling curcumin-loaded liquid crystals in in vitro performance tests. Arabian Journal of Chemistry, 10(7), 1029–1037. https://doi.org/10.1016/j.arabjc.2016.01.014

Khursheed, R., Singh, S. K., Kapoor, B., Gulati, M., Wadhwa, S., Gupta, S., Prasher, P., Kumar, D., Dua, K., Kumar, L. M. S., Babu, U. v., Sharma, M., Soni, H. K., & Kumar, V. (2021). Development and Validation of RP-HPLC Method for Simultaneous Determination of Curcumin and Quercetin in Extracts, Marketed Formulations, and Self-Nanoemulsifying Drug Delivery System. Re:GEN Open, 1(1), 43–52. https://doi.org/10.1089/regen.2021.0021

Gupta, T., Singh, J., Kaur, S., Sandhu, S., Singh, G., & Kaur, I. P. (2020). Enhancing Bioavailability and Stability of Curcumin Using Solid Lipid Nanoparticles (CLEN): A Covenant for Its Effectiveness. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.00879

Yun, C. I., Yang, H. J., Lee, J. H., & Kim, Y. J. (2025). Validation, measurement uncertainty, and application of sesquiterpenoids and curcuminoids in turmeric (Curcuma longa L.) using HPLC-DAD and UHPLC-ESI-MS/MS. Food Science and Biotechnology, 34(3), 611–620. https://doi.org/10.1007/s10068-024-01677-y

Ayurveda Limited Athani, K. (2018). Pharmacognostic Evaluation of Curcuma longa L. Rhizome and Standardization of its Formulation by HPLC Using Curcumin as Marker. Available Online on Www.Ijppr.Com International Journal of Pharmacognosy and Phytochemical Research, 10(1), 38–42. https://doi.org/10.25258/phyto.10.1.7

kadam Associate Professor of Pharmacognosy, P., yadav, K., Kadam, P. v, Yadav, K. N., Bhingare, C. L., & Patil, M. J. (2018). Standardization and quantification of curcumin from Curcuma longa extract using UV visible spectroscopy and HPLC. ~ 1913 ~ Journal of Pharmacognosy and Phytochemistry, 7(5), 1913–1918. https://www.phytojournal.com/archives/2018/vol7issue5/PartAG/7-5-12-325.pdf

Panigrahi, S., & Hirlekar, R. (2016). A new stability-indicating RP-HPLC method for determination of curcumin: An application to nanoparticulate formulation. International Journal of Pharmacy and Pharmaceutical Sciences, 8(12), 149–155. https://doi.org/10.22159/ijpps.2016v8i12.14473

Antunes, A. H., Faria, F. R., Mota, J. F., Santiago, M. F., Kogawa, A. C., & Rezende, K. R. (2020). Bioanalytical method by HPLC-FLD for curcumin analysis in supplemented athletes. Saudi Pharmaceutical Journal, 28(5), 599–606. https://doi.org/10.1016/j.jsps.2020.03.012

Paudel, D., Koirala, S., Pokharel, P., Bhandari, D. R., Adhikari, A., & Pokhrel, M. R. (2025). Optimization and Validation of a New HPLC Method for the Comparative Analysis of Curcumin in Fresh Rhizome Across Gandaki Province, Nepal, and Marketed Turmeric Powder. Journal of Food Quality, 2025(1). https://doi.org/10.1155/jfq/8925770

Praveena, J., & Guru, B. R. (2021). Simultaneous estimation of paclitaxel and curcumin in nano-formulation: Stability analysis of drugs, optimization and validation of HPLC method. Journal of Applied Pharmaceutical Science, 11(3), 071-083,. https://doi.org/10.7324/JAPS.2021.110308

Vaz, G., Clementino, A., Mitsou, E., Ferrari, E., Buttini, F., Sissa, C., Xenakis, A., Sonvico, F., & Dora, C. (2022). In Vitro Evaluation of Curcumin- and Quercetin-Loaded Nanoemulsions for Intranasal Administration: Effect of Surface Charge and Viscosity. Pharmaceutics, 14(1). https://doi.org/10.3390/pharmaceutics14010194

Japar Sodik, J., Indra Dinata, D., Herni Kusriani, R., & Jafar, G. (2023). COMPARISON OF ANALYSIS METHODS FOR CURCUMIN DETERMINATION: A LITERATURE REVIEW. In Medical Sains : Jurnal Ilmiah Kefarmasian (Vol. 8, Issue 4). https://doi.org/10.37874/ms.v8i4.850

Sravani, A. B., Mathew, E. M., Ghate, V., & Lewis, S. A. (2022). A Sensitive Spectrofluorimetric Method for Curcumin Analysis. Journal of Fluorescence, 32(4), 1517–1527. https://doi.org/10.1007/s10895-022-02947-w

Rasheed, N. M. A., Srividya, G. S., & Nagaiah, K. (2017). HPTLC method development and quantification of curcumin content in different extracts of rhizomes of Curcuma longa L. Annals of Phytomedicine: An International Journal, 6(2). https://doi.org/10.21276/ap.2017.6.2.6

Abdel-Kader, M. S., Salkini, A. A., Alam, P., Alshahrani, K. A., Foudah, A. I., & Alqarni, M. H. (2022). A High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Curcumin I, Curcumin II and Curcumin III in Curcuma longa and Herbal Formulation. Separations, 9(4). https://doi.org/10.3390/separations9040094

Vera Nanda, E., & Pratiwi, Y. (2021). Validasi Dan Penetapan Kadar Kurkumin Pada Jamu Gendong Kunyit Asam Dengan Metode Kromatografi Cair Kinerja Tinggi Validation and Determination of Curcumin Levels in Tamarind Turmeric Gendong Herbs from the Kemiri Muka Market, Depok with High-Performance Liquid Chromatography (HPLC) Method. In Daerah Khusus Ibukota Jakarta (Vol. 14, Issue 1). https://journal.istn.ac.id/index.php/saintechfarma/article/view/932/666

DOI: https://doi.org/10.52447/inrpj.v10i2.9201